Hotspot

trnM(CAU)-psbD

LSC · 33865-35190 · 1326 bp

Back to Forsythia

Pi 0.000862
MarkerSeek score 54.40
Primer available yes
Label trnM(CAU)-psbD

Feature Metadata

label_name
trnM(CAU)-psbD
feature_type
igs
region
LSC
length_bp
1326
variable_sites
3
pi
0.000862
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM(CAU)-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1485-1486 bp 1.0 79.0
trnM(CAU)-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1488-1489 bp 1.0 78.8
trnM(CAU)-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1475-1476 bp 1.0 77.9
trnM(CAU)-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1478-1479 bp 1.0 77.7
trnM(CAU)-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1462-1463 bp 1.0 77.2