Hotspot

trnE(UUC)-trnM(CAU)

LSC · 32985-33792 · 808 bp

Back to Forsythia

Pi 0.00188

MarkerSeek score 51.80

Primer available yes

Label trnE(UUC)-trnM(CAU)

Feature Metadata

label_name: trnE(UUC)-trnM(CAU)
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE(UUC)-trnM(CAU)_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	925-949 bp	1.0	80.9
trnE(UUC)-trnM(CAU)_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	904-928 bp	1.0	80.5
trnE(UUC)-trnM(CAU)_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	901-925 bp	1.0	80.3
trnE(UUC)-trnM(CAU)_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	887-911 bp	1.0	80.2
trnE(UUC)-trnM(CAU)_p5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	922-946 bp	1.0	79.8