Hotspot

psbM-trnD(GUC)

LSC · 31508-32579 · 1072 bp

Back to Forsythia

Pi 0.00191

MarkerSeek score 52.00

Primer available yes

Label psbM-trnD(GUC)

Feature Metadata

label_name: psbM-trnD(GUC)
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 6
pi: 0.00191
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD(GUC)_p1	AGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.4	58.5	1197-1209 bp	1.0	56.4
psbM-trnD(GUC)_p2	AGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.4	58.5	1189-1201 bp	1.0	56.2
psbM-trnD(GUC)_p3	AAGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.2	58.5	1190-1202 bp	1.0	54.7
psbM-trnD(GUC)_p4	AGCGGTAGGAACTAGAATGAA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.2	58.5	1189-1201 bp	1.0	54.7
psbM-trnD(GUC)_p5	AAGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.1	58.5	1198-1210 bp	1.0	54.2