Hotspot

trnC(GCA)-petN

LSC · 29331-30158 · 828 bp

Back to Forsythia

Pi 0.00311
MarkerSeek score 56.80
Primer available yes
Label trnC(GCA)-petN

Feature Metadata

label_name
trnC(GCA)-petN
feature_type
igs
region
LSC
length_bp
828
variable_sites
8
pi
0.00311
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC(GCA)-petN_p1 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 931-938 bp 1.0 80.6
trnC(GCA)-petN_p2 TTTCCCCAGTTCAAATCCGG TTCTTCCCCACACTACGAGT 50.0 50.0 58.1 58.0 929-936 bp 1.0 80.6
trnC(GCA)-petN_p3 GTATCATTTTGGCGGCATGG ACTTCTTCCCCACACTACGA 50.0 50.0 57.9 58.0 983-990 bp 1.0 80.1
trnC(GCA)-petN_p4 GTATCATTTTGGCGGCATGG TTCTTCCCCACACTACGAGT 50.0 50.0 57.9 58.0 981-988 bp 1.0 80.1
trnC(GCA)-petN_p5 TTTCCCCAGTTCAAATCCGG CACTTCTTCCCCACACTACG 50.0 55.0 58.1 57.9 932-939 bp 1.0 80.0