Hotspot

rps16-trnQ(UUG)

LSC · 6393-8134 · 1742 bp

Back to Forsythia

Pi 0.00275
MarkerSeek score 57.20
Primer available yes
Label rps16-trnQ(UUG)

Feature Metadata

label_name
rps16-trnQ(UUG)
feature_type
igs
region
LSC
length_bp
1742
variable_sites
12
pi
0.00275
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ(UUG)_p1 CGGATCTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1795-1829 bp 1.0 81.0
rps16-trnQ(UUG)_p2 ATCTGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1792-1826 bp 1.0 81.0
rps16-trnQ(UUG)_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1781-1815 bp 1.0 80.6
rps16-trnQ(UUG)_p4 CCACAACGGATCTGTCCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1801-1835 bp 1.0 79.5
rps16-trnQ(UUG)_p5 GGATCTGTCCTTCAAGTCGC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1794-1828 bp 1.0 78.8