Hotspot

matK-rps16

LSC · 3656-5261 · 1606 bp

Back to Forsythia

Pi 0.00492

MarkerSeek score 54.50

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1606
variable_sites: 20
pi: 0.00492
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CCTGATCATGAGCAAGTGCA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.3	58.2	1779-1802 bp	1.0	78.9
matK-rps16_p2	ACCCTGATCATGAGCAAGTG	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.6	58.2	1781-1804 bp	1.0	77.6
matK-rps16_p3	CCTGATCATGAGCAAGTGCA	GTGCTCAACCTACAGGAACT	50.0	50.0	58.3	57.2	1778-1801 bp	1.0	73.7
matK-rps16_p4	CCCTGATCATGAGCAAGTGC	GGTGCTCAACCTACAGGAAC	55.0	55.0	59.0	58.2	1780-1803 bp	1.0	73.1
matK-rps16_p5	ACCCTGATCATGAGCAAGTG	GTGCTCAACCTACAGGAACT	50.0	50.0	57.6	57.2	1780-1803 bp	1.0	72.4