Hotspot

trnH(GUG)-psbA

LSC · 90-540 · 451 bp

Back to Forsythia

Pi 0.00739

MarkerSeek score 56.10

Primer available yes

Label trnH(GUG)-psbA

Feature Metadata

label_name: trnH(GUG)-psbA
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 9
pi: 0.00739
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH(GUG)-psbA_p1	TTAGTTATGGGCGAACGACG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	582-592 bp	1.0	79.9
trnH(GUG)-psbA_p2	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	528-538 bp	1.0	79.5
trnH(GUG)-psbA_p3	CCTTAATCCACTTGGCCACA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.8	57.9	524-534 bp	1.0	79.1
trnH(GUG)-psbA_p4	TTAGTTATGGGCGAACGACG	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.1	58.3	631-641 bp	1.0	77.7
trnH(GUG)-psbA_p5	TTAGTTATGGGCGAACGACG	ACCGTGCTAACCTTGGTATG	50.0	50.0	58.1	57.6	632-642 bp	1.0	77.3