Hotspot

rps15

SSC · 125620-125892 · 273 bp

Back to Forsskaolea

Pi 0.491

MarkerSeek score 73.70

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 110
pi: 0.491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	ACCCCCTCAACTCGTTCTAA	TTTGTGAGTGTATACGGCCG	50.0	50.0	58.0	58.0	1213 bp	0.5	59.8
rps15_p2	AACACCATGCATTGATGGGT	TTTGTGAGTGTATACGGCCG	45.0	50.0	58.1	58.0	671 bp	0.5	59.5
rps15_p3	CATAAGGTAAATCGGCCGCT	TTTGTGAGTGTATACGGCCG	50.0	50.0	58.1	58.0	1104 bp	0.5	59.0
rps15_p4	CATACCAGTGGCAGCTTCAA	TTTGTGAGTGTATACGGCCG	50.0	50.0	58.2	58.0	1049 bp	0.5	58.6
rps15_p5	CGCCCCAATATCTGCCATAA	TTTGTGAGTGTATACGGCCG	50.0	50.0	57.8	58.0	977 bp	0.5	58.4