Hotspot

psaC

SSC · 119491-119736 · 246 bp

Back to Forsskaolea

Pi 0.492

MarkerSeek score 73.40

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 98
pi: 0.492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TCTTTCCTCCCCACATGGAT	CCAATTCAATGCGTTGCCTT	50.0	45.0	58.0	57.9	1506 bp	0.5	59.2
psaC_p2	AACGTTTCTTTCCTCCCCAC	CCAATTCAATGCGTTGCCTT	50.0	45.0	58.0	57.9	1512 bp	0.5	59.2
psaC_p3	TCTTTCCTCCCCACATGGAT	GCGGATCCCTAAGTGGATTG	50.0	55.0	58.0	58.1	995 bp	0.5	59.0
psaC_p4	AACGTTTCTTTCCTCCCCAC	GCGGATCCCTAAGTGGATTG	50.0	55.0	58.0	58.1	1001 bp	0.5	58.9
psaC_p5	TCTTTCCTCCCCACATGGAT	AATGCGTTGCCTTGTTGTTC	50.0	45.0	58.0	58.1	1499 bp	0.5	58.7