Hotspot

ndhI

SSC · 121477-122052 · 576 bp

Back to Forsskaolea

Pi 0.453

MarkerSeek score 81.20

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 576
variable_sites: 229
pi: 0.453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TAAATAAGGAGTTGGGCCGC	AGCCGTATGTGGTGGAAATC	50.0	50.0	58.0	58.0	1799 bp	0.5	59.3
ndhI_p2	TAAATAAGGAGTTGGGCCGC	GAGCCGTATGTGGTGGAAAT	50.0	50.0	58.0	58.0	1800 bp	0.5	59.3
ndhI_p3	TAAATAAGGAGTTGGGCCGC	GCCGTATGTGGTGGAAATCT	50.0	50.0	58.0	58.0	1798 bp	0.5	59.3
ndhI_p4	CCCATTACCAACGGTCCAAA	AGCCGTATGTGGTGGAAATC	50.0	50.0	58.1	58.0	1896 bp	0.5	59.0
ndhI_p5	CCCATTACCAACGGTCCAAA	GCCGTATGTGGTGGAAATCT	50.0	50.0	58.1	58.0	1895 bp	0.5	59.0