Hotspot

ndhH

SSC · 124335-125516 · 1182 bp

Back to Forsskaolea

Pi 0.478

MarkerSeek score 80.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 470
pi: 0.478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GATATGACCGCTATGGCTGG	TTTGTGAGTGTATACGGCCG	55.0	50.0	58.0	58.0	2102 bp	0.5	59.9
ndhH_p2	GGATATGACCGCTATGGCTG	TTTGTGAGTGTATACGGCCG	55.0	50.0	58.0	58.0	2103 bp	0.5	59.9
ndhH_p3	ACTCAATTCATTCCGCCCTC	TTTGTGAGTGTATACGGCCG	50.0	50.0	58.0	58.0	2412 bp	0.5	59.6
ndhH_p4	TGTCTTAACGAATCGCACGT	TTTGTGAGTGTATACGGCCG	45.0	50.0	57.9	58.0	2350 bp	0.5	59.6
ndhH_p5	GATATGACCGCTATGGCTGG	CGCGTTAATTTGACGAGTCG	55.0	50.0	58.0	58.0	1617 bp	0.5	59.6