Hotspot

ndhG

SSC · 120546-121076 · 531 bp

Back to Forsskaolea

Pi 0.442

MarkerSeek score 79.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 197
pi: 0.442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AAGGCAACGCATTGAATTGG	AAATTGCCCTGGGTCGTTTA	45.0	45.0	57.9	57.7	1199 bp	0.5	57.0
ndhG_p2	CAATCCACTTAGGGATCCGC	AAATTGCCCTGGGTCGTTTA	55.0	45.0	58.1	57.7	1710 bp	0.5	56.7
ndhG_p3	GAACAACAAGGCAACGCATT	AAATTGCCCTGGGTCGTTTA	45.0	45.0	58.1	57.7	1206 bp	0.5	56.4
ndhG_p4	AAGGCAACGCATTGAATTGG	TCAACAGCTGATTGGTTGGT	45.0	45.0	57.9	57.6	997 bp	0.5	56.4
ndhG_p5	AAGGCAACGCATTGAATTGG	CTACCCGTCGTTGATTGGAA	45.0	50.0	57.9	57.6	1383 bp	0.5	56.3