Hotspot

ndhF

SSC · 111701-113971 · 2271 bp

Back to Forsskaolea

Pi 0.435

MarkerSeek score 79.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2271
variable_sites: 859
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGGATCCGGACAAAATCGA	GTGCCATAGTTTGACGACCA	50.0	50.0	57.4	58.2	2979 bp	0.5	53.7
ndhF_p2	GAGGATCCGGACAAAATCGA	AAAGTGAAGTCTTGGGTGCC	50.0	50.0	57.4	58.3	2994 bp	0.5	52.8
ndhF_p3	GAGGATCCGGACAAAATCGA	TTGGGTGCCATAGTTTGACG	50.0	50.0	57.4	58.5	2983 bp	0.5	51.5
ndhF_p4	GAGGATCCGGACAAAATCGA	GTGAAGTCTTGGGTGCCATA	50.0	50.0	57.4	57.5	2991 bp	0.5	51.3
ndhF_p5	GAGGATCCGGACAAAATCGAT	GTGCCATAGTTTGACGACCA	47.6	50.0	57.9	58.2	2979 bp	0.5	49.5