Hotspot

ndhD

SSC · 117853-119382 · 1530 bp

Back to Forsskaolea

Pi 0.463

MarkerSeek score 80.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 568
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCTAATGAAGCCTGGGGATC	TCTGCTCCAAGAACAGAGGA	55.0	50.0	58.1	58.0	2291 bp	0.5	59.1
ndhD_p2	GCTAATGAAGCCTGGGGATC	GGGACGGGTGTAAAGCTAAG	55.0	55.0	58.1	58.0	2320 bp	0.5	59.1
ndhD_p3	GCTAATGAAGCCTGGGGATC	AAGATGTGAATCCGCCTGTC	55.0	50.0	58.1	58.0	2256 bp	0.5	59.0
ndhD_p4	GAAGCCTGGGGATCGTATTG	TCTGCTCCAAGAACAGAGGA	55.0	50.0	58.1	58.0	2285 bp	0.5	59.0
ndhD_p5	GAAGCCTGGGGATCGTATTG	GGGACGGGTGTAAAGCTAAG	55.0	55.0	58.1	58.0	2314 bp	0.5	58.9