Hotspot

ndhA

SSC · 122059-124333 · 2275 bp

Back to Forsskaolea

Pi 0.483

MarkerSeek score 77.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2275
variable_sites: 884
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TAAACGACCCAGGGCAATTT	AGAGTTGAAGCCCCAAAAGG	45.0	50.0	57.7	58.0	2987 bp	0.5	57.6
ndhA_p2	TAAACGACCCAGGGCAATTT	AGTTGAAGCCCCAAAAGGAG	45.0	50.0	57.7	58.0	2985 bp	0.5	57.6
ndhA_p3	TAAACGACCCAGGGCAATTT	GAGTTGAAGCCCCAAAAGGA	45.0	50.0	57.7	58.0	2986 bp	0.5	57.6
ndhA_p4	TAAACGACCCAGGGCAATTT	GTTGAAGCCCCAAAAGGAGA	45.0	50.0	57.7	58.0	2984 bp	0.5	57.6
ndhA_p5	TAAACGACCCAGGGCAATTT	GTGAGAGTTGAAGCCCCAAA	45.0	50.0	57.7	58.0	2990 bp	0.5	57.5