Hotspot

ccsA

SSC · 116585-117550 · 966 bp

Back to Forsskaolea

Pi 0.429

MarkerSeek score 77.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 302
pi: 0.429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GCGGAATGGCTATCACACTT	50.0	50.0	58.0	58.3	1814 bp	0.5	57.3
ccsA_p2	TGAAATCGGTAGACACGCTG	GCGGAATGGCTATCACACTT	50.0	50.0	58.0	58.3	1813 bp	0.5	57.3
ccsA_p3	GTGAAATCGGTAGACACGCT	TCAAGCGAGAACCCTTTGTT	50.0	45.0	58.0	57.7	1251 bp	0.5	57.2
ccsA_p4	TGAAATCGGTAGACACGCTG	TCAAGCGAGAACCCTTTGTT	50.0	45.0	58.0	57.7	1250 bp	0.5	57.2
ccsA_p5	GTGAAATCGGTAGACACGCT	ATGGCCTCTCTTGCATTACC	50.0	50.0	58.0	57.6	1761 bp	0.5	57.1