Hotspot

psaC-ndhE

SSC · 119737-120011 · 275 bp

Back to Forsskaolea

Pi 0.481

MarkerSeek score 78.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 275
variable_sites: 126
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TCCTCTGTTCTTGGAGCAGA	CCAATTCAATGCGTTGCCTT	50.0	45.0	58.0	57.9	790 bp	0.5	59.2
psaC-ndhE_p2	CTTAGCTTTACACCCGTCCC	CCAATTCAATGCGTTGCCTT	55.0	45.0	58.0	57.9	761 bp	0.5	59.2
psaC-ndhE_p3	GACAGGCGGATTCACATCTT	CCAATTCAATGCGTTGCCTT	50.0	45.0	58.0	57.9	825 bp	0.5	59.1
psaC-ndhE_p4	TCGAGTTGTTTCATGCCACA	CCAATTCAATGCGTTGCCTT	45.0	45.0	58.0	57.9	875 bp	0.5	59.0
psaC-ndhE_p5	TTACACCCGTCCCAAGGTAT	CCAATTCAATGCGTTGCCTT	50.0	45.0	58.0	57.9	754 bp	0.5	59.0