Hotspot

rpl32-trnL-UAG

SSC · 115403-116439 · 1037 bp

Back to Forsskaolea

Pi 0.431

MarkerSeek score 80.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1037
variable_sites: 438
pi: 0.431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGAAAGGGTATTGGGCTGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1162 bp	0.5	58.8
rpl32-trnL-UAG_p2	AAGAAAGGGTATTGGGCTGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1163 bp	0.5	58.7
rpl32-trnL-UAG_p3	GGGCTGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1149 bp	0.5	58.5
rpl32-trnL-UAG_p4	GGGCTGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1150 bp	0.5	58.5
rpl32-trnL-UAG_p5	GAAAGGGTATTGGGCTGCAT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1160 bp	0.5	58.2