Hotspot

ndhF-rpl32

SSC · 113972-115243 · 1272 bp

Back to Forsskaolea

Pi 0.429

MarkerSeek score 82.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1272
variable_sites: 488
pi: 0.429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCGAAGATCTTTTGTTGCC	GCAGCCCAATACCCTTTCTT	50.0	50.0	58.0	58.1	1467 bp	0.5	58.7
ndhF-rpl32_p2	GGCGAAGATCTTTTGTTGCC	AAAAGCTTTCAATGCAGCCC	50.0	45.0	58.0	57.8	1480 bp	0.5	58.5
ndhF-rpl32_p3	CTCCGATGCGATTGGTTACA	GCAGCCCAATACCCTTTCTT	50.0	50.0	58.1	58.1	1911 bp	0.5	58.3
ndhF-rpl32_p4	GATTTTGCAATCGGACCTGC	GCAGCCCAATACCCTTTCTT	50.0	50.0	58.1	58.1	2077 bp	0.5	58.1
ndhF-rpl32_p5	GGCGAAGATCTTTTGTTGCC	ATGCAGCCCAATACCCTTTC	50.0	50.0	58.0	58.2	1469 bp	0.5	58.1