primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score ndhF_p1 ndhF 1 GAGGATCCGGACAAAATCGA GTGCCATAGTTTGACGACCA 20 20 50.0 50.0 57.4 58.2 6.1 0.0 0.793569 111701 113971 2979-2979 0.500000 53.7 ndhF_p2 ndhF 2 GAGGATCCGGACAAAATCGA AAAGTGAAGTCTTGGGTGCC 20 20 50.0 50.0 57.4 58.3 6.1 0.0 0.904493 111701 113971 2994-2994 0.500000 52.8 ndhF_p3 ndhF 3 GAGGATCCGGACAAAATCGA TTGGGTGCCATAGTTTGACG 20 20 50.0 50.0 57.4 58.5 6.1 0.0 1.067678 111701 113971 2983-2983 0.500000 51.5 ndhF_p4 ndhF 4 GAGGATCCGGACAAAATCGA GTGAAGTCTTGGGTGCCATA 20 20 50.0 50.0 57.4 57.5 6.1 0.0 1.086842 111701 113971 2991-2991 0.500000 51.3 ndhF_p5 ndhF 5 GAGGATCCGGACAAAATCGAT GTGCCATAGTTTGACGACCA 21 20 47.6 50.0 57.9 58.2 6.1 0.0 1.309926 111701 113971 2979-2979 0.500000 49.5 ndhF-rpl32_p1 ndhF-rpl32 1 GGCGAAGATCTTTTGTTGCC GCAGCCCAATACCCTTTCTT 20 20 50.0 50.0 58.0 58.1 9.9 0.0 0.164755 113972 115243 1467-1467 0.500000 58.7 ndhF-rpl32_p2 ndhF-rpl32 2 GGCGAAGATCTTTTGTTGCC AAAAGCTTTCAATGCAGCCC 20 20 50.0 45.0 58.0 57.8 9.9 13.1 0.191751 113972 115243 1480-1480 0.500000 58.5 ndhF-rpl32_p3 ndhF-rpl32 3 CTCCGATGCGATTGGTTACA GCAGCCCAATACCCTTTCTT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.212779 113972 115243 1911-1911 0.500000 58.3 ndhF-rpl32_p4 ndhF-rpl32 4 GATTTTGCAATCGGACCTGC GCAGCCCAATACCCTTTCTT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.234051 113972 115243 2077-2077 0.500000 58.1 ndhF-rpl32_p5 ndhF-rpl32 5 GGCGAAGATCTTTTGTTGCC ATGCAGCCCAATACCCTTTC 20 20 50.0 50.0 58.0 58.2 9.9 0.0 0.236885 113972 115243 1469-1469 0.500000 58.1 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 AAGAAAGGGTATTGGGCTGC AGCGTGTCTACCGATTTCAC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.155198 115403 116439 1162-1162 0.500000 58.8 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 AAGAAAGGGTATTGGGCTGC CAGCGTGTCTACCGATTTCA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.156568 115403 116439 1163-1163 0.500000 58.7 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 GGGCTGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 57.8 58.0 14.0 0.0 0.182194 115403 116439 1149-1149 0.500000 58.5 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 GGGCTGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 20 20 45.0 50.0 57.8 58.0 14.0 0.0 0.183564 115403 116439 1150-1150 0.500000 58.5 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 GAAAGGGTATTGGGCTGCAT AGCGTGTCTACCGATTTCAC 20 20 50.0 50.0 58.2 58.0 0.5 0.0 0.227328 115403 116439 1160-1160 0.500000 58.2 ccsA_p1 ccsA 1 GTGAAATCGGTAGACACGCT GCGGAATGGCTATCACACTT 20 20 50.0 50.0 58.0 58.3 0.0 0.0 0.339411 116585 117550 1814-1814 0.500000 57.3 ccsA_p2 ccsA 2 TGAAATCGGTAGACACGCTG GCGGAATGGCTATCACACTT 20 20 50.0 50.0 58.0 58.3 0.0 0.0 0.340781 116585 117550 1813-1813 0.500000 57.3 ccsA_p3 ccsA 3 GTGAAATCGGTAGACACGCT TCAAGCGAGAACCCTTTGTT 20 20 50.0 45.0 58.0 57.7 0.0 0.0 0.349677 116585 117550 1251-1251 0.500000 57.2 ccsA_p4 ccsA 4 TGAAATCGGTAGACACGCTG TCAAGCGAGAACCCTTTGTT 20 20 50.0 45.0 58.0 57.7 0.0 0.0 0.351047 116585 117550 1250-1250 0.500000 57.2 ccsA_p5 ccsA 5 GTGAAATCGGTAGACACGCT ATGGCCTCTCTTGCATTACC 20 20 50.0 50.0 58.0 57.6 0.0 3.3 0.362840 116585 117550 1761-1761 0.500000 57.1 ndhD_p1 ndhD 1 GCTAATGAAGCCTGGGGATC TCTGCTCCAAGAACAGAGGA 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.112518 117853 119382 2291-2291 0.500000 59.1 ndhD_p2 ndhD 2 GCTAATGAAGCCTGGGGATC GGGACGGGTGTAAAGCTAAG 20 20 55.0 55.0 58.1 58.0 0.0 0.0 0.117010 117853 119382 2320-2320 0.500000 59.1 ndhD_p3 ndhD 3 GCTAATGAAGCCTGGGGATC AAGATGTGAATCCGCCTGTC 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.126900 117853 119382 2256-2256 0.500000 59.0 ndhD_p4 ndhD 4 GAAGCCTGGGGATCGTATTG TCTGCTCCAAGAACAGAGGA 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.131221 117853 119382 2285-2285 0.500000 59.0 ndhD_p5 ndhD 5 GAAGCCTGGGGATCGTATTG GGGACGGGTGTAAAGCTAAG 20 20 55.0 55.0 58.1 58.0 0.0 0.0 0.135712 117853 119382 2314-2314 0.500000 58.9 psaC_p1 psaC 1 TCTTTCCTCCCCACATGGAT CCAATTCAATGCGTTGCCTT 20 20 50.0 45.0 58.0 57.9 0.0 14.0 0.097545 119491 119736 1506-1506 0.500000 59.2 psaC_p2 psaC 2 AACGTTTCTTTCCTCCCCAC CCAATTCAATGCGTTGCCTT 20 20 50.0 45.0 58.0 57.9 0.0 14.0 0.106110 119491 119736 1512-1512 0.500000 59.2 psaC_p3 psaC 3 TCTTTCCTCCCCACATGGAT GCGGATCCCTAAGTGGATTG 20 20 50.0 55.0 58.0 58.1 0.0 6.1 0.130143 119491 119736 995-995 0.500000 59.0 psaC_p4 psaC 4 AACGTTTCTTTCCTCCCCAC GCGGATCCCTAAGTGGATTG 20 20 50.0 55.0 58.0 58.1 0.0 6.1 0.138709 119491 119736 1001-1001 0.500000 58.9 psaC_p5 psaC 5 TCTTTCCTCCCCACATGGAT AATGCGTTGCCTTGTTGTTC 20 20 50.0 45.0 58.0 58.1 0.0 0.0 0.162720 119491 119736 1499-1499 0.500000 58.7 psaC-ndhE_p1 psaC-ndhE 1 TCCTCTGTTCTTGGAGCAGA CCAATTCAATGCGTTGCCTT 20 20 50.0 45.0 58.0 57.9 12.1 14.0 0.098623 119737 120011 790-790 0.500000 59.2 psaC-ndhE_p2 psaC-ndhE 2 CTTAGCTTTACACCCGTCCC CCAATTCAATGCGTTGCCTT 20 20 55.0 45.0 58.0 57.9 0.0 14.0 0.103114 119737 120011 761-761 0.500000 59.2 psaC-ndhE_p3 psaC-ndhE 3 GACAGGCGGATTCACATCTT CCAATTCAATGCGTTGCCTT 20 20 50.0 45.0 58.0 57.9 0.0 14.0 0.113005 119737 120011 825-825 0.500000 59.1 psaC-ndhE_p4 psaC-ndhE 4 TCGAGTTGTTTCATGCCACA CCAATTCAATGCGTTGCCTT 20 20 45.0 45.0 58.0 57.9 0.0 14.0 0.122750 119737 120011 875-875 0.500000 59.0 psaC-ndhE_p5 psaC-ndhE 5 TTACACCCGTCCCAAGGTAT CCAATTCAATGCGTTGCCTT 20 20 50.0 45.0 58.0 57.9 0.0 14.0 0.127715 119737 120011 754-754 0.500000 59.0 ndhE-ndhG_p1 ndhE-ndhG 1 TCCTCTGTTCTTGGAGCAGA GCGGCCCAACTCCTTATTTA 20 20 50.0 50.0 58.0 58.0 12.1 0.0 0.062458 120318 120545 1303-1303 0.500000 59.5 ndhE-ndhG_p2 ndhE-ndhG 2 CTTAGCTTTACACCCGTCCC GCGGCCCAACTCCTTATTTA 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.066949 120318 120545 1274-1274 0.500000 59.5 ndhE-ndhG_p3 ndhE-ndhG 3 TTACACCCGTCCCAAGGTAT GCGGCCCAACTCCTTATTTA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.091551 120318 120545 1267-1267 0.500000 59.3 ndhE-ndhG_p4 ndhE-ndhG 4 TCCTCTGTTCTTGGAGCAGA TTTGGACCGTTGGTAATGGG 20 20 50.0 50.0 58.0 58.1 12.1 4.9 0.103039 120318 120545 1206-1206 0.500000 59.2 ndhE-ndhG_p5 ndhE-ndhG 5 CTTAGCTTTACACCCGTCCC TTTGGACCGTTGGTAATGGG 20 20 55.0 50.0 58.0 58.1 0.0 4.9 0.107531 120318 120545 1177-1177 0.500000 59.1 ndhG_p1 ndhG 1 AAGGCAACGCATTGAATTGG AAATTGCCCTGGGTCGTTTA 20 20 45.0 45.0 57.9 57.7 0.0 15.6 0.379239 120546 121076 1199-1199 0.500000 57.0 ndhG_p2 ndhG 2 CAATCCACTTAGGGATCCGC AAATTGCCCTGGGTCGTTTA 20 20 55.0 45.0 58.1 57.7 5.5 15.6 0.411838 120546 121076 1710-1710 0.500000 56.7 ndhG_p3 ndhG 3 GAACAACAAGGCAACGCATT AAATTGCCCTGGGTCGTTTA 20 20 45.0 45.0 58.1 57.7 0.0 15.6 0.444414 120546 121076 1206-1206 0.500000 56.4 ndhG_p4 ndhG 4 AAGGCAACGCATTGAATTGG TCAACAGCTGATTGGTTGGT 20 20 45.0 45.0 57.9 57.6 0.0 23.3 0.446473 120546 121076 997-997 0.500000 56.4 ndhG_p5 ndhG 5 AAGGCAACGCATTGAATTGG CTACCCGTCGTTGATTGGAA 20 20 45.0 50.0 57.9 57.6 0.0 0.0 0.456707 120546 121076 1383-1383 0.500000 56.3 ndhI_p1 ndhI 1 TAAATAAGGAGTTGGGCCGC AGCCGTATGTGGTGGAAATC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.085389 121477 122052 1799-1799 0.500000 59.3 ndhI_p2 ndhI 2 TAAATAAGGAGTTGGGCCGC GAGCCGTATGTGGTGGAAAT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.085389 121477 122052 1800-1800 0.500000 59.3 ndhI_p3 ndhI 3 TAAATAAGGAGTTGGGCCGC GCCGTATGTGGTGGAAATCT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.085389 121477 122052 1798-1798 0.500000 59.3 ndhI_p4 ndhI 4 CCCATTACCAACGGTCCAAA AGCCGTATGTGGTGGAAATC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.125970 121477 122052 1896-1896 0.500000 59.0 ndhI_p5 ndhI 5 CCCATTACCAACGGTCCAAA GCCGTATGTGGTGGAAATCT 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.125970 121477 122052 1895-1895 0.500000 59.0 ndhA_p1 ndhA 1 TAAACGACCCAGGGCAATTT AGAGTTGAAGCCCCAAAAGG 20 20 45.0 50.0 57.7 58.0 4.9 0.0 0.297149 122059 124333 2987-2987 0.500000 57.6 ndhA_p2 ndhA 2 TAAACGACCCAGGGCAATTT AGTTGAAGCCCCAAAAGGAG 20 20 45.0 50.0 57.7 58.0 4.9 0.0 0.297149 122059 124333 2985-2985 0.500000 57.6 ndhA_p3 ndhA 3 TAAACGACCCAGGGCAATTT GAGTTGAAGCCCCAAAAGGA 20 20 45.0 50.0 57.7 58.0 4.9 0.0 0.300697 122059 124333 2986-2986 0.500000 57.6 ndhA_p4 ndhA 4 TAAACGACCCAGGGCAATTT GTTGAAGCCCCAAAAGGAGA 20 20 45.0 50.0 57.7 58.0 4.9 0.0 0.300697 122059 124333 2984-2984 0.500000 57.6 ndhA_p5 ndhA 5 TAAACGACCCAGGGCAATTT GTGAGAGTTGAAGCCCCAAA 20 20 45.0 50.0 57.7 58.0 4.9 0.0 0.314816 122059 124333 2990-2990 0.500000 57.5 ndhH_p1 ndhH 1 GATATGACCGCTATGGCTGG TTTGTGAGTGTATACGGCCG 20 20 55.0 50.0 58.0 58.0 9.7 26.9 0.016687 124335 125516 2102-2102 0.500000 59.9 ndhH_p2 ndhH 2 GGATATGACCGCTATGGCTG TTTGTGAGTGTATACGGCCG 20 20 55.0 50.0 58.0 58.0 0.0 26.9 0.016687 124335 125516 2103-2103 0.500000 59.9 ndhH_p3 ndhH 3 ACTCAATTCATTCCGCCCTC TTTGTGAGTGTATACGGCCG 20 20 50.0 50.0 58.0 58.0 0.0 26.9 0.045074 124335 125516 2412-2412 0.500000 59.6 ndhH_p4 ndhH 4 TGTCTTAACGAATCGCACGT TTTGTGAGTGTATACGGCCG 20 20 45.0 50.0 57.9 58.0 1.2 26.9 0.055639 124335 125516 2350-2350 0.500000 59.6 ndhH_p5 ndhH 5 GATATGACCGCTATGGCTGG CGCGTTAATTTGACGAGTCG 20 20 55.0 50.0 58.0 58.0 9.7 16.7 0.055946 124335 125516 1617-1617 0.500000 59.6 rps15_p1 rps15 1 ACCCCCTCAACTCGTTCTAA TTTGTGAGTGTATACGGCCG 20 20 50.0 50.0 58.0 58.0 0.0 26.9 0.020759 125620 125892 1213-1213 0.500000 59.8 rps15_p2 rps15 2 AACACCATGCATTGATGGGT TTTGTGAGTGTATACGGCCG 20 20 45.0 50.0 58.1 58.0 8.9 26.9 0.059574 125620 125892 671-671 0.500000 59.5 rps15_p3 rps15 3 CATAAGGTAAATCGGCCGCT TTTGTGAGTGTATACGGCCG 20 20 50.0 50.0 58.1 58.0 23.5 26.9 0.121999 125620 125892 1104-1104 0.500000 59.0 rps15_p4 rps15 4 CATACCAGTGGCAGCTTCAA TTTGTGAGTGTATACGGCCG 20 20 50.0 50.0 58.2 58.0 9.8 26.9 0.175861 125620 125892 1049-1049 0.500000 58.6 rps15_p5 rps15 5 CGCCCCAATATCTGCCATAA TTTGTGAGTGTATACGGCCG 20 20 50.0 50.0 57.8 58.0 0.0 26.9 0.199779 125620 125892 977-977 0.500000 58.4 rps15-ycf1_p1 rps15-ycf1 1 CGACTCGTCAAATTAACGCG TATTGAAAGGCGCGAACTGA 20 20 50.0 45.0 58.0 57.9 3.5 12.1 0.127711 125893 126331 1337-1337 0.500000 59.0 rps15-ycf1_p2 rps15-ycf1 2 AACACCATGCATTGATGGGT TATTGAAAGGCGCGAACTGA 20 20 45.0 45.0 58.1 57.9 8.9 12.1 0.146124 125893 126331 1503-1503 0.500000 58.8 rps15-ycf1_p3 rps15-ycf1 3 CGACTCGTCAAATTAACGCG TGATACCAACAACGGCAGTC 20 20 50.0 50.0 58.0 58.2 3.5 0.0 0.236876 125893 126331 806-806 0.500000 58.1 rps15-ycf1_p4 rps15-ycf1 4 CGACTCGTCAAATTAACGCG TTCAATTGCGATGGAGTGGT 20 20 50.0 45.0 58.0 57.8 3.5 0.0 0.239037 125893 126331 1443-1443 0.500000 58.1 rps15-ycf1_p5 rps15-ycf1 5 AACACCATGCATTGATGGGT TGATACCAACAACGGCAGTC 20 20 45.0 50.0 58.1 58.2 8.9 0.0 0.255289 125893 126331 972-972 0.500000 58.0 ycf1_p1 ycf1 1 GACTGCCGTTGTTGGTATCA TTTTGCCCGTTGGTTTTTCC 20 20 50.0 45.0 58.2 58.0 0.0 0.0 0.222254 126817 126936 1031-1031 0.500000 58.2 ycf1_p2 ycf1 2 GACTGCCGTTGTTGGTATCA CGCAGTCCAAGCAGATTTTG 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.223528 126817 126936 809-809 0.500000 58.2 ycf1_p3 ycf1 3 GACTGCCGTTGTTGGTATCA AGCAATATGGATACGGACGC 20 20 50.0 50.0 58.2 57.9 0.0 0.0 0.276952 126817 126936 1231-1231 0.500000 57.8 ycf1_p4 ycf1 4 ACTGCCGTTGTTGGTATCAA TTTTGCCCGTTGGTTTTTCC 20 20 45.0 45.0 57.7 58.0 0.0 0.0 0.289594 126817 126936 1030-1030 0.500000 57.7 ycf1_p5 ycf1 5 ACTGCCGTTGTTGGTATCAA CGCAGTCCAAGCAGATTTTG 20 20 45.0 50.0 57.7 58.0 0.0 0.0 0.290867 126817 126936 808-808 0.500000 57.7