Hotspot

ycf1

SSC · 124789-130413 · 5625 bp

Back to Forestiera

Pi 0.00644

MarkerSeek score 36.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 90
pi: 0.00644
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACGATACTTTTGATAACCCA	CCTTTTGGTTCTCCCCGAAA	33.3	50.0	52.9	58.0	180 bp	1.0	43.6
ycf1_p2	ACGATACTTTTGATAACCCAT	CCTTTTGGTTCTCCCCGAAA	33.3	50.0	52.6	58.0	179 bp	1.0	43.6
ycf1_p3	ACGATACTTTTGATAACCCATT	CCTTTTGGTTCTCCCCGAAA	31.8	50.0	53.5	58.0	179 bp	1.0	43.6
ycf1_p4	AAACGATACTTTTGATAACCCA	CCTTTTGGTTCTCCCCGAAA	31.8	50.0	53.7	58.0	181 bp	1.0	43.5
ycf1_p5	AAAACGATACTTTTGATAACCCA	CCTTTTGGTTCTCCCCGAAA	30.4	50.0	54.5	58.0	182 bp	1.0	43.5