Hotspot

rpoA

LSC · 79730-80743 · 1014 bp

Back to Forestiera

Pi 0.00289

MarkerSeek score 51.70

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 8
pi: 0.00289
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGTGCTAAAGATTCAAAACCT	GTTGCAGACCCCCGAAAAA	33.3	52.6	53.6	58.6	1184-1189 bp	1.0	40.7
rpoA_p2	TTGTGCTAAAGATTCAAAACCT	GTTGCAGACCCCCGAAAAA	31.8	52.6	54.5	58.6	1185-1190 bp	1.0	40.7
rpoA_p3	TGAATTGTGCTAAAGATTCAAAACC	GTTGCAGACCCCCGAAAAA	32.0	52.6	57.1	58.6	1189-1194 bp	1.0	40.7
rpoA_p4	ATTGTGCTAAAGATTCAAAACCT	GTTGCAGACCCCCGAAAAA	30.4	52.6	55.0	58.6	1186-1191 bp	1.0	40.7
rpoA_p5	TGTGCTAAAGATTCAAAACCT	GTTGCAGACCCCCGAAAA	33.3	55.6	53.6	57.8	1184-1189 bp	1.0	40.7