Hotspot

ndhF

SSC · 111511-113727 · 2217 bp

Back to Forestiera

Pi 0.00496

MarkerSeek score 41.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 28
pi: 0.00496
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCCGAAGAAGATCCTTCTCC	AACGCTGTCCCATATGCTTT	55.0	45.0	57.8	57.9	2995-3000 bp	0.833333	71.5
ndhF_p2	CGCCGAAGAAGATCCTTCTC	ACGCTGTCCCATATGCTTTC	55.0	50.0	58.2	58.3	2995-3000 bp	0.833333	70.5
ndhF_p3	CGCCGAAGAAGATCCTTCTC	GCTGTCCCATATGCTTTCCT	55.0	50.0	58.2	57.6	2993-2998 bp	0.833333	70.3
ndhF_p4	GCCGAAGAAGATCCTTCTCC	ACGCTGTCCCATATGCTTTC	55.0	50.0	57.8	58.3	2994-2999 bp	0.833333	69.8
ndhF_p5	CGCCGAAGAAGATCCTTCTC	AACGCTGTCCCATATGCTTT	55.0	45.0	58.2	57.9	2996-3000 bp	0.666667	64.9