Hotspot

ndhF-rpl32

SSC · 113728-114203 · 476 bp

Back to Forestiera

Pi 0.00938

MarkerSeek score 40.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 476
variable_sites: 12
pi: 0.00938
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TGCTTTTAACGCTGTCCCAT	50.0	45.0	58.0	58.1	666-669 bp	1.0	81.3
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	ATGCTTTTAACGCTGTCCCA	50.0	45.0	58.0	58.1	667-670 bp	1.0	81.2
ndhF-rpl32_p3	TGCCGTCGGAAAAAGAAGAA	ATGCTTTTAACGCTGTCCCA	45.0	45.0	58.0	58.1	665-668 bp	1.0	81.2
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	TGCTTTTAACGCTGTCCCAT	45.0	45.0	58.0	58.1	664-667 bp	1.0	81.2
ndhF-rpl32_p5	TTGCCGTCGGAAAAAGAAGA	TGCTTTTAACGCTGTCCCAT	45.0	45.0	58.0	58.1	665-668 bp	1.0	81.2