Hotspot

rpl22-rps19

LSC · 85495-85560 · 66 bp

Back to Forestiera

Pi 0.0141

MarkerSeek score 53.00

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 2
pi: 0.0141
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TAGGAGCGTCCACGAATTTG	GCGATAATAGATCTCGGCGT	50.0	50.0	58.0	57.5	187-194 bp	1.0	78.9
rpl22-rps19_p2	AGCGTCCACGAATTTGATCA	GCGATAATAGATCTCGGCGT	45.0	50.0	57.9	57.5	183-190 bp	1.0	78.3
rpl22-rps19_p3	TTTCCTCATAGGAGCGTCCA	GCGATAATAGATCTCGGCGT	50.0	50.0	58.1	57.5	195-202 bp	1.0	77.7
rpl22-rps19_p4	CATAGGAGCGTCCACGAATT	GCGATAATAGATCTCGGCGT	50.0	50.0	57.8	57.5	189-196 bp	1.0	77.1
rpl22-rps19_p5	GTTTCCTCATAGGAGCGTCC	GCGATAATAGATCTCGGCGT	55.0	50.0	57.8	57.5	196-203 bp	1.0	77.0