Hotspot

rpl33-rps18

LSC · 69680-69852 · 173 bp

Back to Forestiera

Pi 0.00886

MarkerSeek score 51.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 4
pi: 0.00886
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GGCACAATACGCCTAATCGA	CAATTCGATCCCCCGATTGA	50.0	50.0	58.1	57.7	341-344 bp	1.0	78.5
rpl33-rps18_p2	GGCACAATACGCCTAATCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	342-345 bp	1.0	78.5
rpl33-rps18_p3	ACTCAAAAGAACCGGCACAA	CAATTCGATCCCCCGATTGA	45.0	50.0	58.2	57.7	354-357 bp	1.0	77.6
rpl33-rps18_p4	ACTCAAAAGAACCGGCACAA	TCAATTCGATCCCCCGATTG	45.0	50.0	58.2	57.7	355-358 bp	1.0	77.6
rpl33-rps18_p5	GGCACAATACGCCTAATCGA	TTCGATCCCCCGATTGAATC	50.0	50.0	58.1	57.5	338-341 bp	1.0	76.2