Hotspot

petA-psbJ

LSC · 64783-65733 · 951 bp

Back to Forestiera

Pi 0.00666

MarkerSeek score 51.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 17
pi: 0.00666
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAATTGGCCGAAATGAATTTCT	ATTCCGGATTGGGTTCATCC	36.4	50.0	56.2	57.6	1000-1006 bp	1.0	48.1
petA-psbJ_p2	ATTGGCCGAAATGAATTTCT	ATTCCGGATTGGGTTCATCC	35.0	50.0	53.8	57.6	998-1004 bp	1.0	45.0
petA-psbJ_p3	CAATTGGCCGAAATGAATTTC	ATTCCGGATTGGGTTCATCC	38.1	50.0	54.7	57.6	1000-1006 bp	1.0	44.3
petA-psbJ_p4	AATTGGCCGAAATGAATTTCT	ATTCCGGATTGGGTTCATCC	33.3	50.0	54.7	57.6	999-1005 bp	1.0	44.0
petA-psbJ_p5	TGGCCGAAATGAATTTCTAG	ATTCCGGATTGGGTTCATCC	40.0	50.0	53.7	57.6	996-1002 bp	1.0	44.0