Hotspot

atpB-rbcL

LSC · 56551-57334 · 784 bp

Back to Forestiera

Pi 0.0151

MarkerSeek score 56.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 22
pi: 0.0151
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGGCCCAGGTTTTGTTT	AACACCCGCTTTGAATCCAA	45.0	45.0	58.0	58.3	902-905 bp	1.0	79.6
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	873-876 bp	1.0	79.0
atpB-rbcL_p3	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	944-947 bp	1.0	78.3
atpB-rbcL_p4	TACGGCCCAGGTTTTGTTTT	AACACCCGCTTTGAATCCAA	45.0	45.0	58.2	58.3	901-904 bp	1.0	77.9
atpB-rbcL_p5	GGACCGATGATTTGGACGAT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	920-923 bp	1.0	77.7