Hotspot

psaA-ycf3

LSC · 43520-44255 · 736 bp

Back to Forestiera

Pi 0.00408

MarkerSeek score 52.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 6
pi: 0.00408
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	895-896 bp	1.0	79.3
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	847-848 bp	1.0	78.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCAGCTGA	50.0	45.0	57.9	57.7	920-921 bp	1.0	77.5
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGGCTATAGCGCTTACTCCT	50.0	50.0	57.9	57.7	889-890 bp	1.0	77.3
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTGGAAACAGGCTATAGCGC	50.0	50.0	57.9	58.3	897-898 bp	1.0	77.0