Hotspot

psbZ-trnG

LSC · 37877-38165 · 289 bp

Back to Forestiera

Pi 0.0164

MarkerSeek score 47.90

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 289
variable_sites: 10
pi: 0.0164
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	GGGTTAGTCTTTCTGGTGGG	AATCGAACCCGCATCTTCTC	55.0	50.0	57.5	58.1	391-394 bp	1.0	78.7
psbZ-trnG_p2	GGGTTAGTCTTTCTGGTGGG	GAATCGAACCCGCATCTTCT	55.0	50.0	57.5	58.1	392-395 bp	1.0	78.6
psbZ-trnG_p3	GGGTTAGTCTTTCTGGTGGG	GAACCCGCATCTTCTCCTTG	55.0	55.0	57.5	58.6	387-390 bp	1.0	74.1
psbZ-trnG_p4	GGGTTAGTCTTTCTGGTGGG	TACCATTCGACCATATCCGC	55.0	50.0	57.5	57.3	353-356 bp	1.0	73.6
psbZ-trnG_p5	AGTCTTTCTGGTGGGTATCCT	AATCGAACCCGCATCTTCTC	47.6	50.0	57.8	58.1	386-389 bp	1.0	73.0