Hotspot

trnC-petN

LSC · 29259-30028 · 770 bp

Back to Forestiera

Pi 0.00372

MarkerSeek score 54.80

Primer available yes

Label trnC-petN

Feature Metadata

label_name: trnC-petN
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 7
pi: 0.00372
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	868-886 bp	1.0	81.5
trnC-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	866-884 bp	1.0	81.5
trnC-petN_p3	GTATCATTTTGGCGGCATGG	ACTTCTTCCCCACACTACGA	50.0	50.0	57.9	58.0	920-938 bp	1.0	80.9
trnC-petN_p4	GTATCATTTTGGCGGCATGG	TTCTTCCCCACACTACGAGT	50.0	50.0	57.9	58.0	918-936 bp	1.0	80.9
trnC-petN_p5	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	869-887 bp	1.0	80.9