Hotspot

trnS-trnG

LSC · 9406-10101 · 696 bp

Back to Forestiera

Pi 0.0069

MarkerSeek score 51.10

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 12
pi: 0.0069
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	CAATCCGACGCTTTAGTCCA	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.0	56.6	798-803 bp	1.0	62.5
trnS-trnG_p2	AATCCGACGCTTTAGTCCAC	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.0	56.6	797-802 bp	1.0	62.5
trnS-trnG_p3	ATCAACGGAACCGGAAAGAG	AGACCGAAAGACCCTTTAACT	50.0	42.9	57.9	56.6	862-867 bp	1.0	61.6
trnS-trnG_p4	CATCAACGGAACCGGAAAGA	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.2	56.6	863-868 bp	1.0	60.8
trnS-trnG_p5	TCATCAACGGAACCGGAAAG	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.2	56.6	864-869 bp	1.0	60.8