Hotspot

trnK-rps16

LSC · 4401-5288 · 888 bp

Back to Forestiera

Pi 0.00661

MarkerSeek score 56.10

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 888
variable_sites: 16
pi: 0.00661
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	CGAATTCCTCGCTTCATCCA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.1	58.2	1060-1073 bp	1.0	80.1
trnK-rps16_p2	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	1012-1025 bp	1.0	79.3
trnK-rps16_p3	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	1011-1024 bp	1.0	79.3
trnK-rps16_p4	TCTAGCCGCACTTAAAAGCC	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.3	58.2	1026-1039 bp	1.0	78.5
trnK-rps16_p5	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.3	58.2	1021-1034 bp	1.0	78.4