Hotspot

trnH-psbA

LSC · 92-536 · 445 bp

Back to Forestiera

Pi 0.0114

MarkerSeek score 52.80

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 11
pi: 0.0114
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	TTAGTTATGGGCGAACGACG	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.1	58.3	621-634 bp	1.0	78.6
trnH-psbA_p2	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	566-579 bp	1.0	78.5
trnH-psbA_p3	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	567-580 bp	1.0	78.3
trnH-psbA_p4	TTAGTTATGGGCGAACGACG	ACCGTGCTAACCTTGGTATG	50.0	50.0	58.1	57.6	622-635 bp	1.0	78.2
trnH-psbA_p5	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.9	57.6	567-580 bp	1.0	78.0