Hotspot

ycf1

SSC · 125223-130682 · 5460 bp

Back to Fordiophyton

Pi 0.00786

MarkerSeek score 49.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5460
variable_sites: 191
pi: 0.00786
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTAATTCTAATTGTGTTGCTT	AATCCGATTTCCGCAGAGAC	26.1	50.0	52.6	58.1	203 bp	1.0	47.5
ycf1_p2	TTCTTAATTCTAATTGTGTTGCT	AATCCGATTTCCGCAGAGAC	26.1	50.0	52.6	58.1	204 bp	1.0	47.5
ycf1_p3	TCTTAATTCTAATTGTGTTGCTT	CAATCCGATTTCCGCAGAGA	26.1	50.0	52.6	58.1	204 bp	1.0	47.5
ycf1_p4	TTCTTAATTCTAATTGTGTTGCT	CAATCCGATTTCCGCAGAGA	26.1	50.0	52.6	58.1	205 bp	1.0	47.4
ycf1_p5	TCTTAATTCTAATTGTGTTGCTT	CGATTTCCGCAGAGACATCA	26.1	50.0	52.6	58.1	199 bp	0.909091	43.6