Hotspot

rps15

SSC · 124625-124888 · 264 bp

Back to Fordiophyton

Pi 0.012

MarkerSeek score 47.80

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 14
pi: 0.012
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TGCATTGATGGGTGATGAGG	ATCCCCCGTTGAAAATTCGT	50.0	45.0	57.9	57.8	789-860 bp	1.0	86.7
rps15_p2	GCATTGATGGGTGATGAGGT	ATCCCCCGTTGAAAATTCGT	50.0	45.0	57.9	57.8	788-859 bp	1.0	86.7
rps15_p3	TAACTGTTCGGTTTTCGGCA	ATCCCCCGTTGAAAATTCGT	45.0	45.0	58.0	57.8	902-973 bp	1.0	85.9
rps15_p4	TTTTCCATCCCTCTGTGCAG	ATCCCCCGTTGAAAATTCGT	50.0	45.0	57.8	57.8	879-950 bp	1.0	84.7
rps15_p5	TCTGTGCACCAATATCTGCC	ATCCCCCGTTGAAAATTCGT	50.0	45.0	58.0	57.8	1120-1177 bp	0.909091	80.0