Hotspot

ndhF-rpl32

SSC · 114214-115105 · 892 bp

Back to Fordiophyton

Pi 0.023

MarkerSeek score 80.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 892
variable_sites: 76
pi: 0.023
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	TGAAAAAGCCTTCAATGCTGT	50.0	38.1	58.0	56.9	931-1076 bp	1.0	73.1
ndhF-rpl32_p2	TGGGAAGTGGAATGAGAGGT	ATGAAAAAGCCTTCAATGCTGT	50.0	36.4	58.0	57.4	932-1077 bp	1.0	69.1
ndhF-rpl32_p3	TGGGAAGTGGAATGAGAGGT	TGAAAAAGCCTTCAATGCTG	50.0	40.0	58.0	55.1	931-1076 bp	1.0	66.4
ndhF-rpl32_p4	TGGGAAGTGGAATGAGAGGT	GAAAAAGCCTTCAATGCTGT	50.0	40.0	58.0	55.0	930-1075 bp	1.0	66.3
ndhF-rpl32_p5	TGGGAAGTGGAATGAGAGGT	AATGAAAAAGCCTTCAATGCTGT	50.0	34.8	58.0	58.0	933-1078 bp	1.0	65.7