Hotspot

rps8-rpl14

LSC · 81393-81724 · 332 bp

Back to Fordiophyton

Pi 0.0253

MarkerSeek score 78.40

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 332
variable_sites: 30
pi: 0.0253
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ACGTCAGCAATAGTGTCTCT	CGATTGCCCGAGAATTGAGA	45.0	50.0	56.3	58.1	390-426 bp	1.0	75.9
rps8-rpl14_p2	ACGTCAGCAATAGTGTCTCT	TTGCCCGAGAATTGAGACAG	45.0	50.0	56.3	57.9	387-423 bp	1.0	75.7
rps8-rpl14_p3	ACGTCAGCAATAGTGTCTCT	GCCCGAGAATTGAGACAGTT	45.0	50.0	56.3	57.9	385-421 bp	1.0	75.7
rps8-rpl14_p4	GTCAGCAATAGTGTCTCTACCC	CGATTGCCCGAGAATTGAGA	50.0	50.0	58.0	58.1	388-424 bp	1.0	73.2
rps8-rpl14_p5	GTCAGCAATAGTGTCTCTACCC	TTGCCCGAGAATTGAGACAG	50.0	50.0	58.0	57.9	385-421 bp	1.0	72.9