Hotspot

rps18-rpl20

LSC · 68936-69266 · 331 bp

Back to Fordiophyton

Pi 0.019

MarkerSeek score 73.20

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 27
pi: 0.019
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AAAGAAGCGAATCGACTGCT	ACAATCTGGACAAGAACCGG	45.0	50.0	57.9	57.8	486-497 bp	0.909091	81.2
rps18-rpl20_p2	AAAGAAGCGAATCGACTGCT	ATCTGGACAAGAACCGGTTG	45.0	50.0	57.9	57.8	483-494 bp	0.909091	81.2
rps18-rpl20_p3	AAAGAAGCGAATCGACTGCT	CAATCTGGACAAGAACCGGT	45.0	50.0	57.9	57.8	485-496 bp	0.909091	81.2
rps18-rpl20_p4	GAAAGAAGCGAATCGACTGC	ATCTGGACAAGAACCGGTTG	50.0	50.0	57.6	57.8	484-495 bp	0.909091	78.5
rps18-rpl20_p5	GAAAGAAGCGAATCGACTGC	CAATCTGGACAAGAACCGGT	50.0	50.0	57.6	57.8	486-497 bp	0.909091	78.4