Hotspot

petA-psbJ

LSC · 63418-64322 · 905 bp

Back to Fordiophyton

Pi 0.0125

MarkerSeek score 75.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 42
pi: 0.0125
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	ATACTCAGGGTTGGGCTCAT	45.0	50.0	57.6	58.1	926-1055 bp	1.0	83.3
petA-psbJ_p2	ACGTGTCCAAGGTCTTTTGT	GTTCATACTCAGGGTTGGGC	45.0	55.0	57.6	58.2	930-1059 bp	1.0	82.1
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	TTCATACTCAGGGTTGGGCT	45.0	50.0	57.6	58.3	929-1058 bp	1.0	81.5
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	GGTTCATACTCAGGGTTGGG	45.0	55.0	57.6	57.6	931-1060 bp	1.0	80.7
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	ATACTCAGGGTTGGGCTCAT	50.0	50.0	57.2	58.1	932-1061 bp	1.0	80.6