Hotspot

rps4-trnT-UGU

LSC · 46359-46767 · 409 bp

Back to Fordiophyton

Pi 0.018
MarkerSeek score 70.70
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
409
variable_sites
37
pi
0.018
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAGACCTGGTAAAGCCCCAA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 463-541 bp 1.0 88.5
rps4-trnT-UGU_p2 TTAGACCTGGTAAAGCCCCA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 464-542 bp 1.0 88.5
rps4-trnT-UGU_p3 AAAATCGCTTCCGGCTCTAG CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 496-574 bp 1.0 88.2
rps4-trnT-UGU_p4 CTAAAATCGCTTCCGGCTCT CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 498-576 bp 1.0 88.2
rps4-trnT-UGU_p5 TCTAAAATCGCTTCCGGCTC CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 499-577 bp 1.0 88.1