Hotspot

psbZ-trnG-UCC

LSC · 36613-36900 · 288 bp

Back to Fordiophyton

Pi 0.0213

MarkerSeek score 74.40

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 28
pi: 0.0213
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GACGGCTGGTCAAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	413-447 bp	1.0	87.1
psbZ-trnG-UCC_p2	CCCGTTGTTTTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	434-468 bp	1.0	86.7
psbZ-trnG-UCC_p3	ACGGCTGGTCAAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.7	57.8	412-446 bp	1.0	85.4
psbZ-trnG-UCC_p4	GTTTTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.3	57.8	428-462 bp	1.0	82.4
psbZ-trnG-UCC_p5	GACGGCTGGTCAAGTAACAA	AACTTAACTCGGGCGGATAG	50.0	50.0	58.1	57.1	448-482 bp	1.0	80.9