Hotspot

trnE-UUC-trnT-GGU

LSC · 31329-31958 · 630 bp

Back to Fordiophyton

Pi 0.0163

MarkerSeek score 77.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 630
variable_sites: 46
pi: 0.0163
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	655-717 bp	1.0	87.0
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	634-696 bp	1.0	86.7
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	631-693 bp	1.0	86.6
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.7	58.2	652-714 bp	1.0	85.9
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.7	58.3	657-719 bp	1.0	84.8