Hotspot

rpoB-trnC-GCA

LSC · 27044-28418 · 1375 bp

Back to Fordiophyton

Pi 0.0111
MarkerSeek score 72.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1375
variable_sites
63
pi
0.0111
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TTCCCTCACTTCCATCCACC CCGGATTTGAACTGGGGAAA 55.0 50.0 59.0 58.1 1429-1479 bp 1.0 78.9
rpoB-trnC-GCA_p2 TCACTTCCATCCACCAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 59.0 58.1 1424-1474 bp 1.0 78.8
rpoB-trnC-GCA_p3 GACATTCCCTCACTTCCATCC CCGGATTTGAACTGGGGAAA 52.4 50.0 58.1 58.1 1433-1483 bp 1.0 78.3
rpoB-trnC-GCA_p4 CATTCCCTCACTTCCATCCA CCGGATTTGAACTGGGGAAA 50.0 50.0 56.9 58.1 1431-1481 bp 1.0 78.1
rpoB-trnC-GCA_p5 ATTCCCTCACTTCCATCCAC CCGGATTTGAACTGGGGAAA 50.0 50.0 56.9 58.1 1430-1480 bp 1.0 78.0