Hotspot

trnS-GCU-trnR-UCU

LSC · 7945-9772 · 1828 bp

Back to Fordiophyton

Pi 0.0129
MarkerSeek score 68.90
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1828
variable_sites
91
pi
0.0129
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1863-1944 bp 1.0 76.2
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1862-1943 bp 1.0 76.2
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA ACATAGAAGAACTTGCGTCCA 50.0 42.9 58.0 57.3 1870-1951 bp 1.0 74.4
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC ACATAGAAGAACTTGCGTCCA 50.0 42.9 58.0 57.3 1869-1950 bp 1.0 74.3
trnS-GCU-trnR-UCU_p5 CAATCCGACGCTTTAGTCCA AAGAACTTGCGTCCAATAGGA 50.0 42.9 58.0 57.3 1864-1945 bp 1.0 74.2