Hotspot

trnH-GUG-psbA

LSC · 76-303 · 228 bp

Back to Fordiophyton

Pi 0.0447

MarkerSeek score 80.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 228
variable_sites: 45
pi: 0.0447
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	322-345 bp	1.0	90.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	336-359 bp	1.0	90.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	335-358 bp	1.0	90.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	337-360 bp	1.0	90.7
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	272-295 bp	1.0	90.4