Hotspot

rpl32-trnL-UAG

SSC · 115277-116116 · 840 bp

Back to Fontanesia

Pi 0.0179

MarkerSeek score 64.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 840
variable_sites: 15
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGAAATCTCTTTCTACCGGG	TCCTAAGAGCAGCGTGTCTA	52.4	50.0	57.8	57.9	938-939 bp	1.0	70.9
rpl32-trnL-UAG_p2	GGGAAATCTCTTTCTACCGGG	AGCAGCGTGTCTACCAATTT	52.4	45.0	57.8	57.8	931-932 bp	1.0	70.4
rpl32-trnL-UAG_p3	GGGAAATCTCTTTCTACCGGG	CCTAAGAGCAGCGTGTCTAC	52.4	55.0	57.8	57.8	937-938 bp	1.0	70.3
rpl32-trnL-UAG_p4	GGGAAATCTCTTTCTACCGGG	CTAAGAGCAGCGTGTCTACC	52.4	55.0	57.8	57.8	936-937 bp	1.0	70.3
rpl32-trnL-UAG_p5	GGGAAATCTCTTTCTACCGGG	AAGATGTCATGCCGCTACTC	52.4	50.0	57.8	57.8	988-989 bp	1.0	70.0