Hotspot

rpl16-rps3

LSC · 85154-85304 · 151 bp

Back to Fontanesia

Pi 0.0199

MarkerSeek score 63.90

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 151
variable_sites: 3
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	CCCTACAAACCATTCGAGCT	45.0	50.0	57.6	57.6	281 bp	1.0	74.5
rpl16-rps3_p2	AAACCAACGAGTCACACACT	GGGTTCCCCTACAAACCATT	45.0	50.0	57.6	57.4	287 bp	1.0	72.7
rpl16-rps3_p3	AAACCAACGAGTCACACACT	CCCCTACAAACCATTCGAGC	45.0	55.0	57.6	58.6	282 bp	1.0	72.7
rpl16-rps3_p4	TCATACTATGGGGCTGGTCT	CCCTACAAACCATTCGAGCT	50.0	50.0	57.2	57.6	322 bp	1.0	71.2
rpl16-rps3_p5	AACGAGTCACACACTAAGCA	CCCTACAAACCATTCGAGCT	45.0	50.0	57.1	57.6	276 bp	1.0	70.6