Hotspot

trnF-GAA-ndhJ

LSC · 50486-51139 · 654 bp

Back to Fontanesia

Pi 0.0154

MarkerSeek score 68.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 654
variable_sites: 10
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	793-796 bp	1.0	80.7
trnF-GAA-ndhJ_p2	CTACATTGGGAATGGTCGGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	810-813 bp	1.0	80.4
trnF-GAA-ndhJ_p3	TGCTACATTGGGAATGGTCG	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	812-815 bp	1.0	80.4
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	757-760 bp	1.0	80.4
trnF-GAA-ndhJ_p5	GGATGGGATGCTACATTGGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.1	58.1	820-823 bp	1.0	80.0