Hotspot

trnE-UUC-trnT-GGU

LSC · 32750-33555 · 806 bp

Back to Fontanesia

Pi 0.00751
MarkerSeek score 63.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
806
variable_sites
6
pi
0.00751
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 940-947 bp 1.0 79.8
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 919-926 bp 1.0 79.3
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 916-923 bp 1.0 79.2
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 902-909 bp 1.0 79.0
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC CCGATGACTTACGCCTTACC 55.0 55.0 57.8 58.2 937-944 bp 1.0 78.6